Isolation and primary structure of urotensin II from the brain of a tetrapod, the frog Rana ridibunda

Biochem Biophys Res Commun. 1992 Oct 30;188(2):578-83. doi: 10.1016/0006-291x(92)91095-8.


A peptide related to urotensin II has been isolated in pure form from an extract of the brain of the European green frog, Rana ridibunda. The primary structure of the peptide was established as Ala-Gly-Asn-Leu-Ser-Glu-Cys-Phe-Trp-Lys-Tyr-Cys-Val and this sequence was confirmed by chemical synthesis. Frog urotensin II contains an additional amino acid residue compared with fish urotensin II peptides but the structure of the cyclic region of the molecule has been fully conserved. The data show that urotensin II is not confined to the caudal neurosecretory system of fish but is present in the central nervous system of a tetrapod.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Brain Chemistry*
  • Chromatography, Gel
  • Chromatography, High Pressure Liquid
  • Indicators and Reagents
  • Molecular Sequence Data
  • Rana ridibunda
  • Urotensins / chemical synthesis
  • Urotensins / chemistry*
  • Urotensins / isolation & purification


  • Indicators and Reagents
  • Urotensins
  • urotensin II