Molecular cloning in Escherichia coli, expression, and nucleotide sequence of the gene for the ethylene-forming enzyme of Pseudomonas syringae pv. phaseolicola PK2

Biochem Biophys Res Commun. 1992 Oct 30;188(2):826-32. doi: 10.1016/0006-291x(92)91131-9.

Abstract

The gene for the ethylene-forming enzyme of Pseudomonas syringae pv. phaseolicola PK2 was found to be encoded by an indigenous plasmid, designated pPSP1. The gene for the ethylene-forming enzyme was cloned and expressed in Escherichia coli JM109. Nucleotide sequence analysis of the clone revealed an open reading frame that encodes 350 amino acids (mol. wt. 39,444). In a comparison with other proteins, the homology score for the entire amino-acid sequence of the ethylene-forming enzyme of Pseudomonas syringae versus ethylene-forming enzymes from plants and 2-oxoglutarate-dependent dioxygenases was low. However, functionally significant regions are conserved.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Cloning, Molecular
  • DNA Probes
  • Escherichia coli / genetics*
  • Gene Expression
  • Genes, Bacterial*
  • Lyases / genetics*
  • Lyases / isolation & purification
  • Lyases / metabolism
  • Molecular Sequence Data
  • Oligonucleotide Probes
  • Plasmids
  • Pseudomonas / enzymology*
  • Pseudomonas / genetics*
  • Recombinant Proteins / isolation & purification
  • Recombinant Proteins / metabolism
  • Restriction Mapping

Substances

  • DNA Probes
  • Oligonucleotide Probes
  • Recombinant Proteins
  • Lyases
  • ethylene forming enzyme

Associated data

  • GENBANK/D13182
  • GENBANK/D13240
  • GENBANK/D13241
  • GENBANK/M96017
  • GENBANK/M96018
  • GENBANK/M96019
  • GENBANK/M96020
  • GENBANK/M96021
  • GENBANK/M96022
  • GENBANK/M96023