[Preparation of 5-(125)iodine-2'-deoxyuridine triphosphate and incorporation of the labeled nucleotide into DNA by polymerase chain reaction]

Bioorg Khim. 1992 Aug;18(8):1104-7.
[Article in Russian]

Abstract

Radioiodine was substituted into deoxyuridine triphosphate by radioiodination of 5-mercuri-2'-deoxyuridine triphosphate. The purified 125I-nucleotide was incorporated into DNA in reactions by the protocol of the polymerase chain reaction. The incorporation of the radioactive nucleotide required added thymidine triphosphate for the synthesis of full length molecules during the one minute elongation period. The iodine substituent in the DNA remained covalently bound during both heat and alkaline denaturation.

Publication types

  • English Abstract

MeSH terms

  • Chromatography, DEAE-Cellulose
  • DNA / chemistry*
  • Deoxyuracil Nucleotides / chemistry
  • Deoxyuracil Nucleotides / isolation & purification*
  • Hot Temperature
  • Hydrogen-Ion Concentration
  • Iodine Radioisotopes
  • Nucleic Acid Denaturation
  • Polymerase Chain Reaction

Substances

  • Deoxyuracil Nucleotides
  • Iodine Radioisotopes
  • deoxyuridine triphosphate
  • DNA