Abstract
We report the existence of a sixth replication arrest site, TerF, that is located within the coding sequences of the rcsC gene, a negative regulator of capsule biosynthesis. The TerF site is oriented to allow transcription of the rcsC gene but prevent DNA replication in the terminus-to-origin direction. Our results demonstrate that the TerF site is functional in both chromosomal and plasmid environments and that the stability of the Tus-TerF protein-DNA complex more closely resembles the plasmid R6K Ter sites than the chromosomal TerB site.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Bacterial Proteins / genetics*
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Base Sequence
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Chromosome Mapping
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DNA Replication*
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Escherichia coli / genetics*
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Escherichia coli Proteins*
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Genes, Bacterial / genetics*
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Genes, Regulator / genetics*
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Molecular Sequence Data
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Multienzyme Complexes*
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Phosphoprotein Phosphatases*
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Plasmids / genetics
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Protein Kinases*
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Regulatory Sequences, Nucleic Acid / genetics*
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Transcription Factors / genetics*
Substances
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Bacterial Proteins
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Escherichia coli Proteins
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Multienzyme Complexes
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Transcription Factors
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rcsC protein, E coli
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Protein Kinases
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Phosphoprotein Phosphatases