Analysis of the effect of aging on the response to hypoxia by cDNA microarray

Mech Ageing Dev. Aug-Sep 2003;124(8-9):941-9. doi: 10.1016/s0047-6374(03)00166-0.


Little is known about the detail of hypoxia-responsive gene expression patterns in advanced age, even though aging is thought to be partially associated with a decreased response to hypoxia. In the present study, we identified several hypoxia-inducible genes and investigated the effect of aging on hypoxic gene expression profiles using cDNA microarray analysis of young/old human diploid fibroblasts. Of 7458 genes in the microarray, we found that genes involved in angiogenesis, defense against oxidative stress, and transcription regulation are severely impaired in senescent cell, which is consistent with the fact that aged cells have attenuated responses to various stimuli.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cells, Cultured
  • Cellular Senescence / genetics*
  • Diploidy
  • Down-Regulation
  • Fibroblasts / metabolism
  • Gene Expression Profiling
  • Gene Expression*
  • Humans
  • Hypoxia / genetics*
  • Neovascularization, Physiologic / genetics
  • Oligonucleotide Array Sequence Analysis*
  • Oxidative Stress / genetics
  • Transcription, Genetic / genetics
  • Up-Regulation