A high-throughput capillary assay for bacterial chemotaxis

J Microbiol Methods. 2003 Oct;55(1):315-9. doi: 10.1016/s0167-7012(03)00112-x.

Abstract

We present a high-throughput capillary assay in order to characterize the chemotactic response of the E. coli bacterium. We measure the number of organisms attracted into an array of 96 capillary tubes containing the attractant L-aspartate. The effect of bacterial concentration on the chemotactic response is reported. Such high-throughput assay can be used to characterize bacterial chemotaxis function of a wide range of biochemical parameters.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Chemotaxis*
  • Colony Count, Microbial
  • Escherichia coli / physiology*