Crystallization and preliminary crystallographic analysis of the importin-beta-SREBP-2 complex

Soo Jae Lee; Toshihiro Sekimoto; Eiki Yamashita; Emi Nagoshi; Atsushi Nakagawa; Hideaki Tanaka; Yoshihiro Yoneda; Tomitake Tsukihara

doi:10.1107/s0907444903018328

Crystallization and preliminary crystallographic analysis of the importin-beta-SREBP-2 complex

Acta Crystallogr D Biol Crystallogr. 2003 Oct;59(Pt 10):1866-8. doi: 10.1107/s0907444903018328. Epub 2003 Sep 19.

Authors

Soo Jae Lee¹, Toshihiro Sekimoto, Eiki Yamashita, Emi Nagoshi, Atsushi Nakagawa, Hideaki Tanaka, Yoshihiro Yoneda, Tomitake Tsukihara

Affiliation

¹ Institute for Protein Research, Osaka University, Japan.

PMID: 14501140
DOI: 10.1107/s0907444903018328

Abstract

The nuclear-transport protein importin-beta mediates the nuclear import of the transcription factor SREBP-2 without requiring adaptor proteins such as importin-alpha. An importin-beta-SREBP-2 HLHZ domain complex was purified and crystallized. The crystals belong to space group P2(1)2(1)2(1) and show diffraction to at least 3.0 A resolution. The unit-cell parameters are a = 101.0, b = 113.2, c = 240.0 A. Structure determination using the MAD or SAD method is under way.

MeSH terms

Animals
Crystallization
Crystallography, X-Ray / methods
DNA-Binding Proteins / biosynthesis
DNA-Binding Proteins / chemistry*
DNA-Binding Proteins / genetics
Humans
Mice
Protein Structure, Tertiary
Recombinant Proteins / biosynthesis
Recombinant Proteins / chemistry
Recombinant Proteins / genetics
Sterol Regulatory Element Binding Protein 2
Transcription Factors / biosynthesis
Transcription Factors / chemistry*
Transcription Factors / genetics
beta Karyopherins / biosynthesis
beta Karyopherins / chemistry*
beta Karyopherins / genetics

Substances

DNA-Binding Proteins
Recombinant Proteins
SREBF2 protein, human
Srebf2 protein, mouse
Sterol Regulatory Element Binding Protein 2
Transcription Factors
beta Karyopherins