P-Glycoprotein efflux reduces the brain concentration of the substance P (NK1 receptor) antagonists SR140333 and GR205171: a comparative study using mdr1a-/- and mdr1a+/+ mice

Behav Pharmacol. 2003 Sep;14(5-6):457-63. doi: 10.1097/01.fbp.0000087734.21047.ae.


Investigation of the antidepressant-like actions of substance P (NK1 receptor) antagonists has been hindered by the few available compounds that bind with high affinity to the rat and mouse NK1 receptor, as these are the most commonly used preclinical species. The best available compounds for such studies are SR140333 and GR205171. However, SR140333 does not penetrate the central nervous system (CNS) after systemic administration, and GR205171 is active only at high doses, where unspecific pharmacological effects occur, so that changes in behaviour cannot be attributed to selective NK1 receptor blockade. These compounds may be substrates for P-glycoprotein (P-gp) and hence are actively excluded from the brain. The present studies used mdr1a-/- mice, a spontaneously occurring mutant that is deficient in P-gp, to examine the CNS penetration of SR140333 and GR205171. Following systemic administration of SR140333 and GR205171 (0.01-10 mg/kg i.v.), considerably higher drug concentrations were achieved in the brains of mdr1a-/- than in mdr1a+/+ mice, and this corresponded with a greater ability to inhibit NK1-agonist-induced behaviours in the mdr1a-/- mutants. Moreover, an NK1-receptor-specific inhibition of aggressive behaviour by GR205171 (10 mg/kg) could be demonstrated in mdr1a-/-, but not mdr1a+/+, mice. These findings suggest that P-gp deficient mice may have useful applications in behavioural pharmacology studies, especially when highly brain-penetrant compounds are not yet available.

MeSH terms

  • ATP Binding Cassette Transporter, Subfamily B, Member 1 / pharmacokinetics*
  • ATP Binding Cassette Transporter, Subfamily B, Member 1 / pharmacology*
  • Animals
  • Blood-Brain Barrier*
  • Brain Chemistry
  • Disease Models, Animal
  • Genes, MDR*
  • Male
  • Mice
  • Mice, Knockout
  • Neurokinin-1 Receptor Antagonists*
  • Piperidines / pharmacokinetics*
  • Quinuclidines / pharmacokinetics*
  • Stereoisomerism
  • Tetrazoles / pharmacokinetics*


  • ATP Binding Cassette Transporter, Subfamily B, Member 1
  • Neurokinin-1 Receptor Antagonists
  • Piperidines
  • Quinuclidines
  • Tetrazoles
  • SR 140333
  • vofopitant