Ozone-induced changes of mRNA levels of beta-1,3-glucanase, chitinase and 'pathogenesis-related' protein 1b in tobacco plants

Plant Mol Biol. 1992 Nov;20(4):673-82. doi: 10.1007/BF00046452.

Abstract

Treatment of the ozone-sensitive tobacco cultivar Bel W3 with an ozone pulse (0.15 microliter/l, 5 h) markedly increased the mRNA level of basic beta-1,3-glucanase and to a lower degree that of basic chitinase. The increase of beta-1,3-glucanase mRNA level occurred within 1 h and showed a transient maximum. Seventeen hours after ozone treatment, the beta-1,3-glucanase mRNA level decreased to lower values. The increase of basic chitinase mRNA level was delayed and was less pronounced than that of beta-1,3-glucanase mRNA. Cultivar Bel B showed only a small increase of beta-1,3-glucanase mRNA level after the same ozone treatment, whereas its basic chitinase mRNA was more strongly induced. Prolonged ozone treatment for 2 days of tobacco Bel W3 led to a persistent level of beta-1,3-glucanase and basic chitinase mRNAs, as well as to an increase of acidic chitinase and 'pathogenesis-related' (PR) 1b mRNA levels. The results indicate that genes so far considered to code for PR proteins may also be involved in the plant response to oxidative stress.

MeSH terms

  • Blotting, Northern
  • Chitinases / genetics*
  • Chitinases / metabolism
  • Gene Expression Regulation*
  • Glucan 1,3-beta-Glucosidase
  • Kinetics
  • Ozone / pharmacology*
  • Plant Proteins / genetics*
  • Plant Proteins / metabolism
  • Plants, Toxic*
  • RNA, Messenger / genetics
  • Tobacco / enzymology
  • Tobacco / genetics*
  • beta-Glucosidase / genetics*
  • beta-Glucosidase / metabolism

Substances

  • Plant Proteins
  • RNA, Messenger
  • pathogenesis-related proteins, plant
  • Ozone
  • Chitinases
  • beta-Glucosidase
  • Glucan 1,3-beta-Glucosidase