Molecular cloning of an alanine aminotransferase from NAD-malic enzyme type C4 plant Panicum miliaceum

Plant Mol Biol. 1992 Nov;20(4):705-13. doi: 10.1007/BF00046455.

Abstract

We have determined the nucleotide sequence of a cDNA encoding AlaAT-2, which is believed to function in the C4-pathway of Panicum miliaceum. An open reading frame (1446 bp) encodes a protein of 482 amino acid residues. The deduced amino acid sequence of AlaAT-2 shows 44.2 and 44.8% homology with the amino acid sequences of AlaATs from rat and human livers, respectively. Northern blot analysis showed that the gene encoding AlaAT-2 in mesophyll and bundle sheath cells was the same and transcribed similarly in the cells. The level of translatable mRNA for AlaAT-2 increased dramatically during greening.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alanine Transaminase / genetics*
  • Alanine Transaminase / metabolism
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blotting, Southern
  • Cloning, Molecular
  • DNA / isolation & purification
  • Humans
  • Light
  • Malate Dehydrogenase / genetics*
  • Malate Dehydrogenase / metabolism
  • Molecular Sequence Data
  • Organ Specificity / genetics
  • Panicum / enzymology
  • Panicum / genetics*
  • Panicum / radiation effects
  • Restriction Mapping
  • Sequence Homology, Amino Acid

Substances

  • DNA
  • Malate Dehydrogenase
  • malate dehydrogenase-(oxaloacetate-decarboxylating) (NAD+)
  • Alanine Transaminase