Evidence for posttranscriptional regulation of the multi K homology domain protein vigilin by a small peptide encoded in the 5' leader sequence

Cell Mol Life Sci. 2003 Aug;60(8):1705-15. doi: 10.1007/s00018-003-3134-4.


Vigilin, a K homology (KH) protein has been found in all eukaryotic species studied. It has a unique structure of 14-15 consecutively arranged KH domains which apparently mediate RNA-protein binding. Cloning and sequencing of the mouse vigilin cDNA confirmed that the amino acid sequences of vertebrate vigilins are highly conserved and contain conserved sequence motifs of nuclear import and export sequences. The human and murine vigilin mRNAs carry two alternatively spliced 5' exons. In the 5' leader region of one of the splice variants, variant 1A, we found an upstream open reading frame (uORF) highly conserved between mouse and human. Here we present for the first time evidence that a 13 amino acid long peptide encoded by this uORF is an inhibitor of vigilin expression operating on a posttranscriptional level. We propose that the two structurally different 5' leader sequences of the human vigilin mRNA are involved in the regulation of vigilin biosynthesis.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • 5' Untranslated Regions / genetics
  • Alternative Splicing
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Carrier Proteins*
  • Cell Line
  • Cell-Free System
  • Cloning, Molecular
  • Conserved Sequence
  • DNA, Complementary / genetics
  • Exons
  • Humans
  • Mice
  • Molecular Sequence Data
  • Mutagenesis, Site-Directed
  • Open Reading Frames
  • Protein Biosynthesis
  • Protein Sorting Signals / genetics
  • RNA, Messenger / genetics
  • RNA-Binding Proteins / chemistry*
  • RNA-Binding Proteins / genetics*
  • RNA-Binding Proteins / metabolism
  • Sequence Homology, Amino Acid
  • Sequence Homology, Nucleic Acid


  • 5' Untranslated Regions
  • Carrier Proteins
  • DNA, Complementary
  • Protein Sorting Signals
  • RNA, Messenger
  • RNA-Binding Proteins
  • high density lipoprotein binding protein