Abstract
The Saccharomyces cerevisiae TPT1 gene plays a role in removing the 2'-phosphate from ligated tRNA during the maturation of pre-tRNA. Here we reported the cloning and characterization of the human TRPT1 gene as a homolog of yeast TPT1. The TRPT1 gene is located at human chromosome 11q13 and encodes a polypeptide of 253 amino acids. BLAST searches with its amino acid sequence revealed the ubiquitous occurrence of TRPT1 homologs and their functional relationships with the presence of the DUF60/KptA domain. Northern analysis demonstrated that the gene is primarily expressed in heart and skeletal muscle, with lower or undetectable levels in other tissues studied. A plasmid-shuffling experiment showed that the human TRPT1 gene could complement the tpt1 mutation in S. cerevisiae
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Cloning, Molecular
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DNA, Complementary / genetics
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Exons
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Gene Expression
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Genes, Fungal*
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Genetic Complementation Test
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Humans
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Introns
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Molecular Sequence Data
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Mutation
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Phosphotransferases (Alcohol Group Acceptor) / chemistry
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Phosphotransferases (Alcohol Group Acceptor) / genetics*
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Phosphotransferases (Alcohol Group Acceptor) / metabolism
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Plasmids / genetics
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Saccharomyces cerevisiae / enzymology
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae Proteins*
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Sequence Homology, Amino Acid
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Species Specificity
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Tumor Protein, Translationally-Controlled 1
Substances
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DNA, Complementary
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Saccharomyces cerevisiae Proteins
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TPT1 protein, human
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Tumor Protein, Translationally-Controlled 1
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Phosphotransferases (Alcohol Group Acceptor)
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TPT1 protein, S cerevisiae