Catalase, but not MnSOD, inhibits glucose deprivation-activated ASK1-MEK-MAPK signal transduction pathway and prevents relocalization of Daxx: hydrogen peroxide as a major second messenger of metabolic oxidative stress

J Cell Biochem. 2003 Oct 1;90(2):304-14. doi: 10.1002/jcb.10619.

Abstract

Overexpression of catalase, but not manganese superoxide dismutase (MnSOD), inhibited glucose deprivation-induced cytotoxicity and c-Jun N-terminal kinase 1 (JNK1) activation in human prostate adenocarcinoma DU-145 cells. Suppression of JNK1 activation by catalase overexpression resulted from inhibition of apoptosis signal-regulating kinase 1 (ASK1) activation by preventing dissociation of thioredoxin (TRX) from ASK1. Overexpression of catalase also inhibited relocalization of Daxx from the nucleus to the cytoplasm as well as association of Daxx with ASK1 during glucose deprivation. Taken together, hydrogen peroxide (H(2)O(2)) rather than superoxide anion (O(2) (*-)) acts as a second messenger of metabolic oxidative stress to activate the ASK1-MAPK/extracellular signal-regulated kinase (ERK) kinase (MEK)-mitogen-activated protein kinase (MAPK) signal transduction pathway.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adaptor Proteins, Signal Transducing
  • Adenocarcinoma / enzymology
  • Adenocarcinoma / pathology
  • Apoptosis
  • Carrier Proteins / metabolism*
  • Catalase / metabolism*
  • Glucose / deficiency
  • Humans
  • Hydrogen Peroxide / pharmacology*
  • Intracellular Signaling Peptides and Proteins*
  • MAP Kinase Kinase Kinase 5
  • MAP Kinase Kinase Kinases / antagonists & inhibitors
  • MAP Kinase Kinase Kinases / metabolism
  • MAP Kinase Signaling System*
  • Male
  • Mitogen-Activated Protein Kinase Kinases / antagonists & inhibitors
  • Mitogen-Activated Protein Kinase Kinases / metabolism
  • Nuclear Proteins / metabolism*
  • Oxidants / pharmacology
  • Oxidative Stress / drug effects*
  • Prostatic Neoplasms / enzymology
  • Prostatic Neoplasms / pathology
  • Protein Transport
  • Second Messenger Systems*
  • Superoxide Dismutase / metabolism*
  • Superoxides / pharmacology
  • Thioredoxins / metabolism
  • Tumor Cells, Cultured

Substances

  • Adaptor Proteins, Signal Transducing
  • Carrier Proteins
  • DAXX protein, human
  • Intracellular Signaling Peptides and Proteins
  • Nuclear Proteins
  • Oxidants
  • Superoxides
  • Thioredoxins
  • Hydrogen Peroxide
  • Catalase
  • Superoxide Dismutase
  • MAP Kinase Kinase Kinase 5
  • MAP Kinase Kinase Kinases
  • MAP3K5 protein, human
  • Mitogen-Activated Protein Kinase Kinases
  • Glucose