A review of soluble c-kit (s-kit) as a novel tumor marker and possible molecular target for the treatment of CNS germinoma

Surg Neurol. 2003 Oct;60(4):321-4; discussion 324-5. doi: 10.1016/s0090-3019(03)00430-0.

Abstract

Background: Although germinomas are the most common central nervous system (CNS) germ cell tumors (GCTs), no specific tumor marker(s) has been identified. In the absence of such a marker, effective treatment planning requires surgical intervention to obtain a histologic diagnosis. The proto-oncogene c-kit is a transmembrane tyrosine kinase receptor that plays a crucial role in the development of germ cells and is aberrantly expressed in a variety of neoplasms. A soluble form of the c-kit (s-kit), composed of only the extracellular domain, has been identified as a functional molecule.

Methods: We immunohistochemically analyzed the distribution of c-kit to determine its expression profile in various histologic subtypes of CNS GCTs. To examine whether s-kit represents a novel clinical marker, its concentration in cerebrospinal fluid (CSF) was assayed by sandwich enzyme-linked immunosorbent assay (ELISA).

Results: On the cell surface of germinomas, c-kit was diffusely positive. Some mature teratoma components were weakly immunoreactive for c-kit; syncytiotrophoblastic giant cells were negative. The level of s-kit was significantly higher in germinoma-containing tumors. The CSF concentration of s-kit was correlated with the clinical course; it was markedly higher in patients with subarachnoid dissemination.

Conclusions: We found that s-kit could be a novel tumor marker for CNS germinomas. In addition, the diffuse expression of c-kit suggests that it may serve as a possible molecular target in the treatment of CNS germinomas.

Publication types

  • Review

MeSH terms

  • Biomarkers, Tumor / analysis*
  • Central Nervous System Neoplasms / chemistry*
  • Central Nervous System Neoplasms / drug therapy
  • Germinoma / chemistry*
  • Germinoma / drug therapy
  • Humans
  • Immunohistochemistry
  • Proto-Oncogene Proteins c-kit / analysis*
  • Proto-Oncogene Proteins c-kit / drug effects
  • Solubility

Substances

  • Biomarkers, Tumor
  • Proto-Oncogene Proteins c-kit