Comparison of measles virus-specific antibody titres as measured by enzyme-linked immunosorbent assay and virus neutralisation assay

Vaccine. 2003 Oct 1;21(27-30):4210-4. doi: 10.1016/s0264-410x(03)00490-0.


We assessed whether measles virus-specific antibody levels in the Dutch population as estimated by an enzyme-linked immunosorbent assay (ELISA) were comparable with estimates by virus neutralisation assay (NT), prompted by a relatively low ELISA seroprevalence in the 10-21-year-old group. We tested 791 sera from individuals aged 2-49 years both in ELISA and NT. Seroprevalence in the 10-21-year-old group was 93.4% (95% confidence interval (CI) 89.5-97.2%) in ELISA versus 97.2% (CI 94.7-99.6%) in NT. There was good agreement between NT and ELISA seroprevalences in the vaccinated 2-9-year-olds and the unvaccinated 22-49-year-olds.

Publication types

  • Comparative Study

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Aging / immunology
  • Antibodies, Viral / analysis
  • Antibodies, Viral / biosynthesis*
  • Child
  • Child, Preschool
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Humans
  • Immunization, Secondary
  • Infant
  • Male
  • Measles virus / immunology*
  • Middle Aged
  • Monitoring, Immunologic
  • Netherlands / epidemiology
  • Neutralization Tests


  • Antibodies, Viral