The temporal relationship between the electromyographic (EMG) and ventilatory thresholds was investigated during incremental exercise performed by eight professional road cyclists. The exercise, performed on a cycloergometer, started at 100 W with successive increments of 26 Wx min(-1) until exhaustion. Gas exchange and the root mean square value of EMG (RMS) from eight lower limb muscles were examined throughout the exercise period. Professional cyclists achieved a maximal oxygen consumption, i.e. VO(2max), of 5.4 (0.5) l x min(-1) [74.6 (2.5) ml x min(-1) x kg(-1), range: 67.8-82.4 ml x min(-1) x kg(-1)] and a maximum power ( W(max)) of 475 (30) W (range: 438-516 W). Our results showed at least the occurrence of a first EMG threshold (EMG(Th1)) in 50% (gastrocnemius lateralis) of the subjects and a second EMG threshold (EMG(Th2)) in 63% (gastrocnemius medialis). EMG(Th1) occurred significantly before the first ventilatory threshold (VT(1)), i.e. at 52 (2)% and 62 (9)% of W(max), respectively. Inversely, no significant difference was observed between the occurrence of EMG(Th2) and the second ventilatory threshold (VT(2)), i.e. at 86 (1)% and 89 (7)% of W(max), respectively. These results suggest that the use of EMG may be a useful non-invasive method for detecting the second ventilatory threshold in most of the muscles involved in cycling exercise.