Utilization efficiency of the oxidized purine nucleotide analogs by DNA polymerase eta

Nucleic Acids Res Suppl. 2003;(3):299-300. doi: 10.1093/nass/3.1.299.

Abstract

To elucidate the behavior of DNA polymerase eta against the oxidized purine nucleotides, we determined the utilization efficiency of 2-hydroxy-dATP and 8-hydroxy-dGTP by the recombinant yeast DNA polymerase eta using the primer extension assay with the synthetic oligonucleotide template-primers, and compared those by DNA polymerase alpha. Results indicate that DNA polymerase eta incorporates 2-hydroxy-dATP opposite template G in addition to template T and 8-hydroxy-dGTP opposite A in addition to C, respectively. Kinetic analysis revealed that the rate of mutation caused by 2-OH-dATP and 8-OH-dGTP with DNA polymerase eta should be much higher than those with DNA polymerase alpha.

MeSH terms

  • Base Sequence
  • DNA-Directed DNA Polymerase / metabolism*
  • Oxidation-Reduction
  • Purine Nucleotides / metabolism*

Substances

  • Purine Nucleotides
  • DNA-Directed DNA Polymerase
  • Rad30 protein