Two CD14 promoter polymorphisms and atopic phenotypes in Czech patients with IgE-mediated allergy

Allergy. 2003 Oct;58(10):1023-6. doi: 10.1034/j.1398-9995.2003.00271.x.


Background: Immunoglobulin E (IgE)-mediated allergy belongs to common chronic disorders resulting from an interaction between both genetic and environmental factors. The gene encoding CD14 is a positional candidate gene for allergic diseases as it is localized on chromosome 5q31.1, a region that is linked to asthma and bronchial hyperresponsiveness. Recently, several polymorphisms in the promoter region of this gene have been associated with atopic phenotypes in various populations.

Methods: We investigated relationship among atopic phenotypes and two polymorphisms [C(-159)T and G(-1359)T] in the promoter of the CD14 gene in the Czech population. Polymerase chain reaction with restriction fragment length polymorphism analyses was used to determine the CD14 genotypes in subjects with IgE-mediated allergic diseases (n = 562) and random controls (n = 320).

Results: The CD14 allele or genotype distributions were similar in patients and control group. However, the frequency of the C allele of the C(-159)T polymorphism was higher in patients with positive skin prick tests for moulds than in patients without reactivity to this antigen (P < 0.002, Pcorr<0.01). In addition, we found that patients with homozygous genotype (GG) of the G(-1359)T polymorphism had marginally lower percentage of positive skin prick tests compared with the other genotypes (P < 0.029, Pcorr > 0.05).

Conclusions: Our study supports the idea that CD14 gene variants may act as disease modifiers of IgE-mediated allergic diseases.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Allergens / blood
  • Allergens / immunology
  • Anaphylaxis / blood
  • Anaphylaxis / immunology
  • Asthma / diagnosis
  • Asthma / genetics
  • Asthma / immunology
  • Case-Control Studies
  • Czech Republic
  • Female
  • Genetic Predisposition to Disease
  • Humans
  • Hypersensitivity, Immediate / diagnosis
  • Hypersensitivity, Immediate / genetics*
  • Hypersensitivity, Immediate / immunology
  • Immunoglobulin E / immunology
  • Lipopolysaccharide Receptors / genetics*
  • Male
  • Phenotype
  • Polymorphism, Genetic*
  • Promoter Regions, Genetic


  • Allergens
  • Lipopolysaccharide Receptors
  • Immunoglobulin E