Species and sex differences of aflatoxin B1-induced glutathione S-transferase placental form in single hepatocytes

Cancer Lett. 1992 Oct 21;66(3):249-54. doi: 10.1016/0304-3835(92)90254-s.

Abstract

Species and sex differences of aflatoxin B1 (AFB1)-induced glutathione S-transferase placental form (GST-P) positive single hepatocytes have been investigated 48 h after an intraperitoneal injection of AFB1 to young male and female Fischer rats (2 mg AFB1/kg body wt) and male Syrian golden hamsters (6 mg AFB1/kg body wt). The presence of GST-P positive hepatocytes was examined by the immunohistochemical method. Male rats formed three times as many AFB1-induced GST-P positive hepatocytes as females. Pretreatment of both male and female rats with an inhibitor of GSH synthesis, buthionine sulfoximine (BSO) (4 mmol/kg body wt), 2 h and 4 h before AFB1 injection increased AFB1-induced GST-P positive hepatocytes by about 120% above the controls. Male hamsters formed several-fold less AFB1-induced GST-P positive hepatocytes than male rats. Pretreatment with BSO did not increase AFB1-induced GST-P positive hepatocytes in hamsters even though it produced an increase in hepatic necrosis. It appears that GSH and GSH S-transferases play an important role in modulating hepatic AFB1-DNA binding and AFB1-induced GST-P positive hepatocytes in rats and hamsters.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Aflatoxin B1 / pharmacology*
  • Animals
  • Cells, Cultured
  • Cricetinae
  • Enzyme Induction
  • Female
  • Glutathione Transferase / biosynthesis*
  • Isoenzymes / biosynthesis*
  • Liver / drug effects
  • Liver / enzymology*
  • Male
  • Mesocricetus
  • Placenta / enzymology
  • Pregnancy
  • Rats
  • Rats, Inbred F344
  • Sex Characteristics

Substances

  • Isoenzymes
  • Aflatoxin B1
  • Glutathione Transferase