Dipeptidyl peptidase-IV expression and activity in human glomerular endothelial cells

Biochem Biophys Res Commun. 2003 Oct 10;310(1):28-31. doi: 10.1016/j.bbrc.2003.08.111.


Glucagon-like peptide-1 (GLP-1), a meal-stimulated gastrointestinal insulinotropic hormone inactivated by dipeptidyl peptidase-IV (DPP-IV), is reduced in type 2 diabetic patients. The present study shows that 2-week exposure of human glomerular endothelial cells to high glucose (22 mM) determines a highly significant increase in DPP-IV activity and mRNA expression, which cannot be entirely accounted for by hyperosmolarity. On the other hand, incubation of purified DPP-IV in a buffer solution added with high glucose does not affect enzyme activity. These results suggest that high glucose increases expression and activity of DPP-IV, possibly contributing to GLP-1 reduction in type 2 diabetic patients.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Buffers
  • Cells, Cultured
  • DNA Primers
  • Dipeptidyl Peptidase 4 / genetics
  • Dipeptidyl Peptidase 4 / metabolism*
  • Endothelial Cells / enzymology
  • Glucagon-Like Peptide 1
  • Glucose / administration & dosage
  • Humans
  • Kidney Glomerulus / cytology
  • Kidney Glomerulus / enzymology*
  • Peptides / antagonists & inhibitors
  • RNA, Messenger / genetics


  • Buffers
  • DNA Primers
  • Peptides
  • RNA, Messenger
  • Glucagon-Like Peptide 1
  • Dipeptidyl Peptidase 4
  • Glucose