Abstract
Rat cortical neurons cultured in conditioned media from human monocyte-derived macrophages (MDM) show increased neuronal protein synthesis, neurite outgrowth, mitogen-activating protein kinase activity, and synaptic function. Neurotrophic properties of human MDM-conditioned media are significantly enhanced by human peripheral nerve and to a more limited extent by CD40 ligand pre-stimulation. Such positive effects of MDM secretions on neuronal function parallel the secretion of brain-derived neurotrophic factor (BDNF). MDM activation cues may serve to balance toxic activities produced during neurodegenerative diseases and thus, under certain circumstances, mitigate neuronal degeneration.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adult
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Animals
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Brachial Plexus / enzymology
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Brachial Plexus / metabolism
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Brachial Plexus / physiology*
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Cell Survival / physiology
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Cells, Cultured
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Cerebral Cortex / cytology
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Cerebral Cortex / enzymology
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Cerebral Cortex / physiology
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Culture Media, Conditioned / metabolism
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Culture Media, Conditioned / pharmacology
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Hippocampus / physiology
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Humans
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Intracellular Fluid / enzymology
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Intracellular Fluid / physiology*
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MAP Kinase Signaling System / physiology
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Macrophage Activation / physiology*
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Macrophages / enzymology
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Macrophages / metabolism*
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Male
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Mitochondria / metabolism
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Mitochondria / physiology
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Nerve Growth Factors / biosynthesis*
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Nerve Growth Factors / physiology
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Neurites / physiology*
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Neurons / cytology
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Neurons / enzymology
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Neurons / physiology
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Phosphoprotein Phosphatases / biosynthesis
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Rats
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Rats, Sprague-Dawley
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Second Messenger Systems / physiology*
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Synaptic Transmission / physiology*
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Up-Regulation / physiology
Substances
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Culture Media, Conditioned
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Nerve Growth Factors
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microtubule-associated protein-2 phosphatase
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Phosphoprotein Phosphatases