Transcriptome analysis of the Pseudomonas aeruginosa response to iron

Arch Microbiol. 2003 Nov;180(5):374-9. doi: 10.1007/s00203-003-0602-z. Epub 2003 Sep 26.

Abstract

To successfully infect humans, Pseudomonas aeruginosa (Pa) must overcome the low iron availability in host tissues. A transcriptome comparison was carried out between iron-starved cells of Pa treated with iron and untreated controls. The present study is the first global analysis of the early transcriptional response of exponentially growing Pa to iron. Approximately 1.3% of the Pa genes displayed > or = 5.0-fold changes in mRNA levels in iron-treated cells. Treatment affected the mRNA levels of many genes required for iron acquisition as well as several genes with relevance to virulence previously known to be regulated by iron. More importantly, the analysis permitted identification of 107 Pa genes whose mRNA levels were not previously known to be affected by iron. These genes are good candidates for mutagenesis studies aimed at identifying novel functions relevant to iron metabolism in Pa. Some of these genes encode predicted siderophore receptors, iron transport systems, TonB-dependent receptors, regulatory proteins, and proteins relevant to virulence. Notably, 49 genes encode hypothetical or conserved hypothetical proteins of unknown function, suggesting that they are involved directly or indirectly in iron metabolism or metabolic adaptation to different iron-availability conditions.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adaptation, Biological
  • Bacterial Outer Membrane Proteins / genetics
  • Gene Expression Profiling / methods
  • Gene Expression Regulation, Bacterial*
  • Genes, Bacterial
  • Heat-Shock Proteins / genetics
  • Iron / metabolism*
  • Iron / pharmacology
  • Membrane Transport Proteins / genetics
  • Molecular Chaperones / genetics
  • Pseudomonas aeruginosa / genetics*
  • Pseudomonas aeruginosa / growth & development
  • Pseudomonas aeruginosa / metabolism*
  • Pseudomonas aeruginosa / pathogenicity
  • RNA, Bacterial / genetics
  • RNA, Bacterial / metabolism
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Receptors, Cell Surface / genetics
  • Transcription, Genetic*
  • Virulence / genetics

Substances

  • Bacterial Outer Membrane Proteins
  • Heat-Shock Proteins
  • Membrane Transport Proteins
  • Molecular Chaperones
  • RNA, Bacterial
  • RNA, Messenger
  • Receptors, Cell Surface
  • siderophore receptors
  • Iron