Cytokine release in healthy donors and patients with chronic granulomatous disease upon stimulation with Aspergillus fumigatus

Scand J Infect Dis. 2003;35(8):482-7. doi: 10.1080/00365540310013009.


The release of tumour necrosis factor-alpha (TNF-alpha), interleukin-6 (IL-6) and IL-10 upon stimulation with non-viable conidia and hyphal fragments from Aspergillus fumigatus was investigated in an ex vivo whole-blood model. In healthy volunteers, high numbers of conidia (between 10(6) and 3 x 10(8)/ml) induced a moderate release of TNF-alpha and IL-6. Hyphal fragments (2.5 x 10(5)/ml) were more potent in stimulating the release of these pro-inflammatory cytokines. Although some IL-10 release was observed upon stimulation with either conidia or hyphal fragments, it was not significantly different from that in unstimulated controls. In comparison, in whole blood obtained from 4 patients with chronic granulomatous disease (CGD), a high release of pro-inflammatory cytokines together with a significantly higher IL-10 release than in the healthy controls was seen after stimulation with A. fumigatus. In conclusion, A. fumigatus can trigger the release of pro-inflammatory cytokines in a human whole-blood system, which is likely to be central to the activation of antifungal defence mechanisms. In contrast, A. fumigatus stimulates a higher release of anti-inflammatory cytokines in CGD patients, which may suggest that a dysregulation between pro- and anti-inflammatory cytokines contributes to the increased susceptibility to invasive aspergillosis in this patient group.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aspergillus fumigatus*
  • Blood Donors
  • Case-Control Studies
  • Cytokines / analysis
  • Cytokines / blood
  • Granulomatous Disease, Chronic / blood*
  • Granulomatous Disease, Chronic / therapy
  • Humans
  • Interleukin-10 / analysis
  • Interleukin-10 / blood*
  • Interleukin-6 / analysis
  • Interleukin-6 / blood*
  • Probability
  • Reference Values
  • Sampling Studies
  • Sensitivity and Specificity
  • Statistics, Nonparametric
  • Tumor Necrosis Factor-alpha / analysis*


  • Cytokines
  • Interleukin-6
  • Tumor Necrosis Factor-alpha
  • Interleukin-10