Susceptibility of primary culture neurons from rats of different ages to encephalomyocarditis (EMC) virus infection

Exp Mol Pathol. 2003 Oct;75(2):160-4. doi: 10.1016/s0014-4800(03)00068-6.


The changes in susceptibility of neurons to the D variant of EMC virus (EMC-D) (10(6) PFU/well) were investigated in developing hippocampal primary cultures from postnatal days of 1, 7, and 56 Fischer 344 rats (P1, P7, and P56) for up to 12 h after infection (12 HAI). The virus titer of primary culture neurons increased at 1 HAI, decreased at 2 HAI, increased at 3 HAI, peaked at 8 HAI, and decreased at 12 HAI in all age groups. The titers at 1 and 8 HAI were lowest in P56 cultures. The virus titer of neurons was always higher than that of culture media, especially at 1 HAI, in P1 cultures, whereas the former was lower than the latter from 2 to 3 HAI in P7 cultures and from 2 to 4 HAI in P56 cultures, respectively. Signals of viral RNA detected by in situ hybridization were first observed in the peripheral cytoplasm of neurons at 1 HAI in P1 and P7 cultures and at 4 HAI in P56 cultures, respectively. The signals spread to a large or whole area of cytoplasm and also to processes thereafter. The number of viral RNA-positive neurons and the amount of signals decreased with age. The present results indicated that the susceptibility of primary culture neurons to EMC-D decreased with age but viral replication still occurred in P56 cultures.

MeSH terms

  • Aging*
  • Animals
  • Antigens, Viral / analysis
  • Culture Techniques
  • Disease Susceptibility
  • Encephalomyocarditis virus / physiology*
  • Hippocampus / cytology
  • In Situ Hybridization
  • Neurons / virology*
  • RNA Probes
  • RNA, Viral / analysis
  • Rats
  • Rats, Inbred F344
  • Virus Replication


  • Antigens, Viral
  • RNA Probes
  • RNA, Viral