Quantification of protodioscin and rutin in asparagus shoots by LC/MS and HPLC methods

J Agric Food Chem. 2003 Oct 8;51(21):6132-6. doi: 10.1021/jf0344587.


A liquid chromatography/mass spectrometry (LC/MS) method with selected ion monitoring was developed and validated to analyze the contents of protodioscin and rutin in asparagus. The distribution of rutin and protodioscin within the shoots was found to vary by location, with the tissue closest to the rhizome found to be a rich source of protodioscin, at an average level of 0.025% tissue fresh weight in the three tested lines, while the upper youngest shoot tissue contained the highest amount of rutin at levels of 0.03-0.06% tissue fresh weight. The lower portions of the asparagus shoots that are discarded during grading and processing should instead be considered a promising source of a new value-added nutraceutical product.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Asparagus Plant / chemistry*
  • Chromatography, High Pressure Liquid / methods*
  • Chromatography, Liquid / methods*
  • Diosgenin / analogs & derivatives*
  • Diosgenin / analysis*
  • Plant Extracts / chemistry
  • Rutin / analysis*
  • Saponins / analysis*
  • Spectrometry, Mass, Electrospray Ionization / methods*


  • Plant Extracts
  • Saponins
  • Rutin
  • protodioscin
  • Diosgenin