Study of antihypertensive mechanism of Tribulus terrestris in 2K1C hypertensive rats: role of tissue ACE activity

Life Sci. 2003 Oct 24;73(23):2963-71. doi: 10.1016/j.lfs.2003.04.002.


Tribulus terrestris is a natural herb used for treating many diseases including hypertension. According to previous reports, aqueous extract of tribulus fruits may have some antihypertensive effect with an unknown mechanism. The present study investigated the antihypertensive mechanism of tribulus in 2K1C hypertensive rats by measurement of circulatory and local ACE activity in aorta, heart, kidney and lung. Four groups of rats were selected; control, sham, operated or hypertensive and tribulus treated hypertensive group. Hypertension was induced using silver clip on renal artery by surgery. Four weeks after surgery, a single daily dose of 10 mg/kg of lyophilized aqueous extract of tribulus fruit were given orally to 2K1C rats for four weeks. ACE activity was determined by high performance liquid chromatography (HPLC). Blood pressure was measured by the tail-cuff method. The systolic blood pressure (SBP) was significantly increased in 2K1C rats compared to control rats. The SBP of tribulus fed hypertensive rats was significantly decreased compared to hypertensive rats. The ACE activity in all tissues of 2K1C rats including: aorta, heart, kidney, lung as well as serum were significantly increased compared to normal rats. The ACE activity in all tissues of tribulus fed hypertensive rats was significantly lower than that of hypertensive rats, which was more pronounced in kidney. These results indicated that there is a negative correlation between consumption of tribulus and ACE activity in serum and different tissues in 2K1C rats.

MeSH terms

  • Angiotensin-Converting Enzyme Inhibitors*
  • Animals
  • Antihypertensive Agents / therapeutic use*
  • Blood Pressure / drug effects
  • Blood Pressure / physiology
  • Disease Models, Animal
  • Hypertension, Renovascular / drug therapy*
  • Hypertension, Renovascular / enzymology
  • Male
  • Peptidyl-Dipeptidase A / metabolism*
  • Phytotherapy*
  • Plant Preparations / therapeutic use
  • Rats
  • Rats, Wistar
  • Tribulus*


  • Angiotensin-Converting Enzyme Inhibitors
  • Antihypertensive Agents
  • Plant Preparations
  • Peptidyl-Dipeptidase A