Isolation of lectins by affinity chromatography with porcine plasma proteins immobilized on agarose

Biosci Biotechnol Biochem. 2003 Sep;67(9):2051-4. doi: 10.1271/bbb.67.2051.

Abstract

To develop a convenient method to isolate lectins, we prepared an affinity gel by coupling plasma proteins with agarose beads under conditions where the pH did not exceed 7.5. The validity of the use of this affinity gel in combination with elution using a hapten saccharide was confirmed by isolation of concanavalin A from Jack bean meal. Successful application of the method was demonstrated by isolation of two novel vegetable lectins from udo (Aralia cordate) and wasabi (Wasabia japonica). The method would be useful to isolate new lectins from various sources including plant and animal tissues.

MeSH terms

  • Amino Acid Sequence
  • Amino Acids / analysis
  • Amino Acids / chemistry
  • Animals
  • Aralia / chemistry
  • Blood Proteins / chemistry*
  • Blood Proteins / metabolism
  • Chromatography, Affinity / methods*
  • Electrophoresis, Polyacrylamide Gel / methods
  • Molecular Sequence Data
  • Plant Lectins / chemistry
  • Plant Lectins / isolation & purification*
  • Plant Lectins / metabolism
  • Sepharose / chemistry*
  • Swine
  • Wasabia / chemistry

Substances

  • Amino Acids
  • Blood Proteins
  • Plant Lectins
  • Sepharose