The effects of dimethylsulfoxide in experimental obstructive jaundice

Acta Chir Belg. 2003 Aug;103(4):392-5. doi: 10.1080/00015458.2003.11679450.


Material and methods: Thirty rats were divided into three groups, as sham, control and DMSO groups. Laparatomy was performed on each animal in the control and DMSO groups and common bile ducts were ligated. Common bile duct was observed but was not ligated for the rats in the sham group. Saline solution injection (1.5 mg/kg/intraperitoneally (i.p.)) was begun on the first day of surgical procedure and repeated once a day for the next 5 days. The same procedure was performed with DMSO (1.5 mg/kg/i.p.) instead of saline in the DMSO group. The rats were sacrificed on the postoperative seventh day, at which time venous blood and liver tissue specimens were taken.

Main outcome measurements: On the 7th postoperative day, the bilirubin, AST, ALT, ALP and GGT levels of the control and DMSO groups were significantly higher in comparison with the sham group (p < 0.01). On the 7th postoperative day, the erythrocyte superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) levels of the control and DMSO groups were significantly lower than those of the sham group (p < 0.01), but there was no statistical difference between the two groups (p > 0.05). Erythrocyte and liver malondialdehyde (MDA) levels in the control and DMSO groups were significantly higher compared with the sham group (p < 0.01). However, the MDA levels were significantly lower in the DMSO group compared to the control group (p < 0.01).

Conclusion: It is stated that free oxygen radicals seem to play a role in the liver tissue injury, secondary to obstructive jaundice. In our experimental study, exogenic DMSO seems to have decreased lipid peroxidation and to have improved some of the parameters of liver tissue injury due to the obstructive jaundice in rats.

MeSH terms

  • Animals
  • Dimethyl Sulfoxide / pharmacology*
  • Free Radical Scavengers / pharmacology*
  • Jaundice, Obstructive / complications
  • Jaundice, Obstructive / metabolism*
  • Jaundice, Obstructive / physiopathology
  • Lipid Peroxidation / physiology
  • Liver Diseases / etiology
  • Liver Diseases / metabolism*
  • Liver Diseases / physiopathology
  • Male
  • Rats
  • Rats, Wistar
  • Superoxides / metabolism*


  • Free Radical Scavengers
  • Superoxides
  • Dimethyl Sulfoxide