High-throughput screening of structural proteomics targets using NMR

FEBS Lett. 2003 Sep 25;552(2-3):207-13. doi: 10.1016/s0014-5793(03)00926-8.


We applied a high-throughput strategy for the screening of targets for structural proteomics of Xanthomonas axonopodis pv citri. This strategy is based on the rapid (1)H-(15)N HSQC NMR analysis of bacterial lysates containing selectively (15)N-labelled heterologous proteins. Our analysis permitted us to classify the 19 soluble candidates in terms of 'foldedness', that is, the extent to which they present a well-folded solution structure, as reflected by the quality of their NMR spectra. This classification allowed us to define a priority list to be used as a guide to select protein candidates for further structural studies.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins / chemistry*
  • Bacterial Proteins / genetics*
  • Cloning, Molecular
  • Gene Expression
  • Genes, Bacterial
  • Molecular Structure
  • Nuclear Magnetic Resonance, Biomolecular / methods*
  • Open Reading Frames
  • Proteomics / methods*
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / genetics
  • Solubility
  • Xanthomonas / chemistry*
  • Xanthomonas / genetics*


  • Bacterial Proteins
  • Recombinant Proteins