Abstract
Several approaches have been used to identify the factors involved in mRNA splicing. None of them, however, comprises a straightforward reversible method for inhibiting the second step of splicing using an external reagent other than a chelator. This investigation demonstrates that the addition of boric acid to an in vitro pre-mRNA splicing reaction causes a dose-dependent reversible inhibition effect on the second step of splicing. The mechanism of action does not involve chelation of several metal ions; hindrance of 3' splice-site; or binding to hSlu7. This study presents a novel method for specific reversible inhibition of the second step of pre-mRNA splicing.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Boric Acids / pharmacology*
-
Boronic Acids / chemistry
-
Boronic Acids / pharmacology
-
Globins / genetics
-
Globins / metabolism
-
Humans
-
In Vitro Techniques
-
Kinetics
-
Metals / metabolism
-
RNA Precursors / genetics*
-
RNA Precursors / metabolism*
-
RNA Splicing / drug effects*
-
RNA Splicing Factors
-
Recombinant Proteins / genetics
-
Recombinant Proteins / metabolism
-
Ribonucleoproteins, Small Nuclear / genetics
-
Ribonucleoproteins, Small Nuclear / metabolism
-
Spliceosomes / drug effects
-
Spliceosomes / metabolism
Substances
-
Boric Acids
-
Boronic Acids
-
Metals
-
RNA Precursors
-
RNA Splicing Factors
-
Recombinant Proteins
-
Ribonucleoproteins, Small Nuclear
-
SLU7 protein, human
-
Globins
-
boric acid