Molecular cloning and characterization of a hypoxia-responsive CITED3 cDNA from grass carp

Comp Biochem Physiol B Biochem Mol Biol. 2003 Oct;136(2):163-72. doi: 10.1016/s1096-4959(03)00224-0.


We have isolated a 1586-bp full-length CITED3 cDNA from grass carp which specifies for a cAMP-responsive element-binding protein/p300-interacting transactivator with glutamic acid (E)/aspartic acid (D)-rich C-terminal domain protein. The cDNA, designated as gcCITED3, has an open reading frame of 762 bp and encodes a protein of 253 amino acids with a predicted molecular mass of 28.3 kDa and pI of 6.4. Pairwise comparison showed that gcCITED3 shares high sequence identity with the CITED3 of zebrafish (94%), chicken (72%) and Xenopus (59%). Northern blot analysis indicated that gcCITED3 is most highly expressed and responsive to hypoxia in the carp kidney. Hypoxic induction was also observed in heart, albeit at a lower level. This is the first report on the isolation of a hypoxia-responsive CITED3 gene from fish.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blotting, Northern
  • Carps / genetics*
  • Cloning, Molecular
  • Fish Proteins / chemistry
  • Fish Proteins / genetics*
  • Gene Expression Profiling
  • Humans
  • Hydrophobic and Hydrophilic Interactions
  • Hypoxia / genetics*
  • Molecular Sequence Data
  • Phylogeny
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Trans-Activators / genetics*


  • Fish Proteins
  • RNA, Messenger
  • Trans-Activators

Associated data

  • GENBANK/AY225852