Efficient transformation of the osmotolerant yeast Zygosaccharomyces rouxii by electroporation

Lenka Pribylova; Hana Sychrova

doi:10.1016/s0167-7012(03)00197-0

Efficient transformation of the osmotolerant yeast Zygosaccharomyces rouxii by electroporation

J Microbiol Methods. 2003 Nov;55(2):481-4. doi: 10.1016/s0167-7012(03)00197-0.

Authors

Lenka Pribylova¹, Hana Sychrova

Affiliation

¹ Department of Membrane Transport, Institute of Physiology AS CR, Videnska 1083, 14220 Prague 4, Czech Republic.

PMID: 14529972
DOI: 10.1016/s0167-7012(03)00197-0

Abstract

A rapid and simple electroporation method to transform osmotolerant yeast Zygosaccharomyces rouxii has been developed and conditions for efficient transformation of mutants derived from different Z. rouxii wild-type strains optimized.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

DNA, Fungal / genetics
DNA, Fungal / metabolism
Electroporation / methods*
Lithium / metabolism
Transformation, Genetic / genetics*
Zygosaccharomyces / genetics*

Substances

DNA, Fungal
Lithium