Efficient transformation of the osmotolerant yeast Zygosaccharomyces rouxii by electroporation

J Microbiol Methods. 2003 Nov;55(2):481-4. doi: 10.1016/s0167-7012(03)00197-0.

Abstract

A rapid and simple electroporation method to transform osmotolerant yeast Zygosaccharomyces rouxii has been developed and conditions for efficient transformation of mutants derived from different Z. rouxii wild-type strains optimized.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA, Fungal / genetics
  • DNA, Fungal / metabolism
  • Electroporation / methods*
  • Lithium / metabolism
  • Transformation, Genetic / genetics*
  • Zygosaccharomyces / genetics*

Substances

  • DNA, Fungal
  • Lithium