Retinyl acetate and all-trans-retinoic acid enhance erythroid colony formation in vitro by circulating human progenitors in an improved serum-free medium

Int J Cell Cloning. 1992 Sep;10(5):286-91. doi: 10.1002/stem.5530100506.

Abstract

Retinyl acetate (RA) dramatically increased the production of early (d16) erythroid colonies in vitro by circulating human progenitor cells growing in an improved serum-free (SF) medium. In the absence of either erythropoietin (Epo) or insulin-like growth factor I (IGF-I), RA alone was able to induce the hemoglobinization of cells in these erythroid colonies. RA synergized with Epo or with IGF-I to yield increased numbers of well-hemoglobinized early colonies. In the presence of defined burst promoting activity (BPA) provided by recombinant human interleukin 3 (rHuIL-3) and hemin, RA and all-trans-retinoic acid (ATRA) were identical with respect to their differentiation-inducing function for early erythroid colonies. ATRA increased the number of these colonies in a concentration-dependent manner, with maximal stimulation (3.5-fold) occurring at 30 nM in the presence of 5.5 ng/ml IL-3, 0.1 mM hemin, 3.0 U/ml Epo and 30 nM IGF-I. This appears to be the first demonstration of erythropoietic activity of two metabolic derivatives of vitamin A in SF medium.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cells, Cultured
  • Colony-Forming Units Assay
  • Culture Media, Serum-Free
  • Diterpenes
  • Erythrocytes / drug effects*
  • Erythroid Precursor Cells / cytology
  • Erythroid Precursor Cells / drug effects*
  • Erythropoiesis / drug effects*
  • Erythropoietin / pharmacology
  • Hemin / pharmacology
  • Humans
  • Insulin-Like Growth Factor I / pharmacology
  • Interleukin-3 / pharmacology
  • Recombinant Proteins / pharmacology
  • Retinyl Esters
  • Tretinoin / pharmacology*
  • Vitamin A / analogs & derivatives*
  • Vitamin A / pharmacology

Substances

  • Culture Media, Serum-Free
  • Diterpenes
  • Interleukin-3
  • Recombinant Proteins
  • Retinyl Esters
  • Erythropoietin
  • Vitamin A
  • retinol acetate
  • Tretinoin
  • Insulin-Like Growth Factor I
  • Hemin