Petroleum pollution bioremediation using water-insoluble uric acid as the nitrogen source

Appl Environ Microbiol. 2003 Oct;69(10):6337-9. doi: 10.1128/AEM.69.10.6337-6339.2003.


The biodegradation of hydrocarbon pollutants in open systems is limited by the availability of a utilizable nitrogen source. This limitation can be overcome by using uric acid. Enrichment cultures grown on crude oil-uric acid media yielded mixed and pure cultures that degraded petroleum. In a simulated open system, uric acid bound to crude oil and was available for bacterial growth and petroleum biodegradation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acinetobacter / growth & development*
  • Acinetobacter / metabolism
  • Biodegradation, Environmental
  • Culture Media
  • Nitrogen / metabolism*
  • Petroleum*
  • Substrate Specificity
  • Uric Acid / chemistry
  • Uric Acid / metabolism*
  • Water Pollutants, Chemical / metabolism


  • Culture Media
  • Petroleum
  • Water Pollutants, Chemical
  • Uric Acid
  • Nitrogen