Evaluation of the TRUGENE HCV 5'NC genotyping kit with the new GeneLibrarian module 3.1.2 for genotyping of hepatitis C virus from clinical specimens

Jeffrey J Germer; David W Majewski; Michael Rosser; Amber Thompson; P Shawn Mitchell; Thomas F Smith; Slava Elagin; Joseph D C Yao

doi:10.1128/JCM.41.10.4855-4857.2003

Evaluation of the TRUGENE HCV 5'NC genotyping kit with the new GeneLibrarian module 3.1.2 for genotyping of hepatitis C virus from clinical specimens

J Clin Microbiol. 2003 Oct;41(10):4855-7. doi: 10.1128/JCM.41.10.4855-4857.2003.

Authors

Jeffrey J Germer¹, David W Majewski, Michael Rosser, Amber Thompson, P Shawn Mitchell, Thomas F Smith, Slava Elagin, Joseph D C Yao

Affiliation

¹ Division of Clinical Microbiology, Mayo Clinic, Rochester, Minnesota 55905, USA.

Abstract

The TRUGENE HCV 5'NC genotyping kit (GeneLibrarian modules 3.1.1 and 3.1.2) and VERSANT HCV genotyping assay were compared by using 96 hepatitis C virus (HCV) RNA-positive patient specimens, including HCV genotypes 1, 2, 3, 4, 5, 6, and 10. The TRUGENE HCV 5'NC genotyping kit (GeneLibrarian module 3.1.2) yielded the most accurate genotyping results.

Publication types

Comparative Study
Evaluation Study

MeSH terms

5' Untranslated Regions / genetics*
Databases, Genetic
Genotype
Hepacivirus / classification*
Hepacivirus / genetics
Hepatitis C / virology
Humans
RNA-Dependent RNA Polymerase
Reagent Kits, Diagnostic*
Viral Nonstructural Proteins / genetics

Substances

5' Untranslated Regions
Reagent Kits, Diagnostic
Viral Nonstructural Proteins
NS-5 protein, hepatitis C virus
RNA-Dependent RNA Polymerase