[The cloning of fragments of the streptomycete plasmid pSG1912 as a part of the vector pUC19]

V B Zaverukha; B V Polevoda; L V Polishchuk; B P Matseliukh

[The cloning of fragments of the streptomycete plasmid pSG1912 as a part of the vector pUC19]

Mikrobiol Zh (1978). 1992 Sep-Oct;54(5):30-5.

[Article in Russian]

Authors

V B Zaverukha, B V Polevoda, L V Polishchuk, B P Matseliukh

PMID: 1453989

Abstract

DNA fragments of plasmid pSG1912 isolated from Streptomyces globisporus 1912 have been cloned into vector pUC19 in the E. coli cells. Stability of inheritance of hybrid derivatives in enterobacteria has been studied. The clones where recombinant plasmids are stably inherited have been chosen. The detailed restriction maps of cloned DNA fragments of pSG1912 have been constructed.

MeSH terms

Animals
Chick Embryo
Cloning, Molecular / methods*
DNA, Bacterial / analysis
DNA, Bacterial / genetics
Electrophoresis, Agar Gel
Escherichia coli / genetics
Genetic Vectors / genetics*
Plasmids / genetics*
Recombination, Genetic / genetics
Restriction Mapping
Soil Microbiology
Streptomyces / genetics*

Substances

DNA, Bacterial