Polymerase chain reaction (PCR) techniques for site-directed mutagenesis

Biotechnol Adv. 1992;10(4):535-47. doi: 10.1016/0734-9750(92)91451-j.


Polymerase chain reaction (PCR) technology has revolutionized the process of isolating and amplifying segments of DNA. One powerful application of PCR is its use in precise site-directed mutagenesis (SDM). SDM provides an elegant tool for scientists and engineers to explore biocatalytic mechanisms and processes to understand the structural-functional relationships of enzymes and other proteins. This article reviews techniques and methodology used in site-directed mutagenesis of genes by PCR.