Schizosaccharomyces pombe Rdh54 (TID1) acts with Rhp54 (RAD54) to repair meiotic double-strand breaks

Mol Biol Cell. 2003 Nov;14(11):4707-20. doi: 10.1091/mbc.e03-05-0288. Epub 2003 Aug 7.

Abstract

We report the characterization of rdh54+, the second fission yeast Schizosaccharomyces pombe Rad54 homolog. rdh54+ shares sequence and functional homology to budding yeast RDH54/TID1. Rdh54p is present during meiosis with appropriate timing for a meiotic recombination factor. It interacts with Rhp51 and the meiotic Rhp51 homolog Dmc1 in yeast two-hybrid assays. Deletion of rdh54+ has no effect on DNA damage repair during the haploid vegetative cell cycle. In meiosis, however, rdh54Delta shows decreased spore viability and homologous recombination with a concomitant increase in sister chromatid exchange. The rdh54Delta single mutant repairs meiotic breaks with similar timing to wild type, suggesting redundancy of meiotic recombination factors. Consistent with this, the rdh54Delta rhp54Delta double mutant fails to repair meiotic double strand breaks. Live cell analysis shows that rdh54Delta rhp54Delta asci do not arrest, but undergo both meiotic divisions with near normal timing, suggesting that failure to repair double strand breaks in S. pombe meiosis does not result in checkpoint arrest.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Cell Survival
  • Cloning, Molecular
  • DNA / genetics*
  • DNA Damage / genetics*
  • DNA Helicases
  • DNA Repair / genetics*
  • DNA Repair Enzymes
  • DNA Topoisomerases
  • Meiosis
  • Molecular Sequence Data
  • Mutation
  • Recombination, Genetic / genetics
  • Saccharomyces cerevisiae Proteins / genetics
  • Saccharomyces cerevisiae Proteins / metabolism*
  • Schizosaccharomyces / genetics
  • Schizosaccharomyces / metabolism*
  • Sequence Analysis, Protein
  • Two-Hybrid System Techniques

Substances

  • Saccharomyces cerevisiae Proteins
  • DNA
  • RAD54 protein, S cerevisiae
  • DNA Helicases
  • DNA Topoisomerases
  • RDH54 protein, S cerevisiae
  • DNA Repair Enzymes