Map-based cloning of a gene controlling omega-3 fatty acid desaturation in Arabidopsis

Science. 1992 Nov 20;258(5086):1353-5. doi: 10.1126/science.1455229.

Abstract

A gene from the flowering plant Arabidopsis thaliana that encodes an omega-3 desaturase was cloned on the basis of the genetic map position of a mutation affecting membrane and storage lipid fatty acid composition. Yeast artificial chromosomes covering the genetic locus were identified and used to probe a seed complementary DNA library. A complementary DNA clone for the desaturase was identified and introduced into roots of both wild-type and mutant plants by Ti plasmid-mediated transformation. Transgenic tissues of both mutant and wild-type plants had significantly increased amounts of the fatty acid produced by this desaturase.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Arabidopsis / genetics*
  • Chromosome Mapping
  • Cloning, Molecular
  • Fatty Acid Desaturases / genetics*
  • Genes, Plant*
  • Genetic Complementation Test
  • Molecular Sequence Data
  • Plant Proteins / genetics

Substances

  • Plant Proteins
  • Fatty Acid Desaturases
  • omega-3 fatty acid desaturase