Comparative analyses of Saccharomyces cerevisiae RNAs using Agilent RNA 6000 Nano Assay and agarose gel electrophoresis

FEMS Yeast Res. 2003 Oct;4(1):119-22. doi: 10.1016/S1567-1356(03)00145-4.

Abstract

Precise quantification and quality characterisation of isolated RNAs are prerequisites for their further exploitation in genome-wide microarrays, Northern blots, cDNA library preparation and others. Our data indicate that RNA analyses using Agilent RNA Nano Assay exhibit several advantages when compared with those performed on ethidium bromide-stained agarose gel electrophoresis or on a spectrophotometer. The RNA Nano Assay makes it possible to estimate RNA concentrations in the range from 1000 ng microl(-1) to 17 ng microl(-1). The presence of impurities including traces of DNA within RNA samples does not influence the concentration measurements. Like agarose gel electrophoresis, RNA Nano Assay allows to analyse RNAs dissolved in formamide and therefore protected against RNase action. Moreover, it allows a clearer distinction of partially degraded samples. The limitation of RNA Nano Assay is the impossibility to detect and to analyse double-stranded RNAs.

Publication types

  • Comparative Study
  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Electrophoresis, Agar Gel / methods*
  • Gene Expression / genetics
  • Polymerase Chain Reaction / instrumentation
  • Polymerase Chain Reaction / methods
  • RNA, Fungal / analysis*
  • Reagent Kits, Diagnostic*
  • Saccharomyces cerevisiae / genetics*

Substances

  • RNA, Fungal
  • Reagent Kits, Diagnostic