A juvenile form of postsynaptic hippocampal long-term potentiation in mice deficient for the AMPA receptor subunit GluR-A

J Physiol. 2003 Dec 15;553(Pt 3):843-56. doi: 10.1113/jphysiol.2003.053637. Epub 2003 Oct 10.


In adult mice, long-term potentiation (LTP) of synaptic transmission at CA3-to-CA1 synapses induced by tetanic stimulation requires L-alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA) receptors containing GluR-A subunits. Here, we report a GluR-A-independent form of LTP, which is comparable in size to LTP in wild-type mice at postnatal day 14 (P14) but diminishes between P14 and P42 in brain slices of GluR-A-deficient mice. The GluR-A-independent form of LTP is sensitive to D(-)-2-amino-5-phosphonopentanoic acid (D-AP5), but lacks short-term potentiation (STP) and can also be observed in the pairing induction protocol. As judged by unaltered paired-pulse facilitation, this LTP form is postsynaptically expressed despite depleted extrasynaptic AMPA receptor pools with reduced levels of GluR-B, which accumulates in somata and synapses of CA1 pyramidal neurons in GluR-A-deficient mice. Our results show that in the developing hippocampus synaptic plasticity can be expressed by AMPA receptors lacking the GluR-A subunit.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Crosses, Genetic
  • DNA Primers
  • Excitatory Postsynaptic Potentials / physiology*
  • Hippocampus / physiology*
  • In Vitro Techniques
  • Long-Term Potentiation / physiology*
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Microscopy, Immunoelectron
  • Protein Subunits / genetics
  • Pyramidal Cells / physiology
  • Receptors, AMPA / deficiency*
  • Receptors, AMPA / genetics
  • Receptors, AMPA / physiology*
  • Reference Values
  • Synaptic Transmission


  • DNA Primers
  • Protein Subunits
  • Receptors, AMPA
  • glutamate receptor ionotropic, AMPA 1