Identification of new partners of the epithelial sodium channel alpha subunit

C R Biol. 2003 Jul;326(7):615-24. doi: 10.1016/s1631-0691(03)00154-9.


A fine regulation of the amiloride-sensitive Epithelial Sodium Channel (ENaC), made of alpha, beta and gamma subunits, is crucial for maintenance of Na+ balance and blood pressure. Both beta- and gamma-ENaC participate in negative regulation by interacting with Nedd4-2, an E3 ubiquitin-ligase. Disruption of this interaction results in increased ENaC activity (Liddle syndrome). By two-hybrid screenings, we identified new potential partners of alpha-ENaC: WWP1 (E3 ubiquitin-ligase protein), UBC9 and TSG101 (E2 ubiquitin/SUMO-conjugating enzymes) and confirmed these interactions in GST pull-down assays. All these partners are implicated in protein trafficking and could be involved in the regulation of ENaC activity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Epithelial Sodium Channels
  • Humans
  • Molecular Sequence Data
  • Protein Structure, Tertiary
  • Sodium Channels / analysis*
  • Sodium Channels / physiology


  • Epithelial Sodium Channels
  • SCNN1B protein, human
  • Sodium Channels