Egr-1 target genes in human endothelial cells identified by microarray analysis

Gene. 2003 Oct 2:315:33-41. doi: 10.1016/s0378-1119(03)00730-3.


Early growth response factor 1 (Egr-1) is a key transcriptional factor to mediate gene expression after vascular injury. To better understand the role of Egr-1 in vasculature, we globally profiled Egr-1 target genes in human endothelial cells using adenoviral gene transfer and Affymetrix oligonucleotide-based microarray technology. More than 300 genes regulated by >/=3-fold with Egr-1 overexpression were identified and, partially, confirmed by Northern and Western blotting, including genes for transcriptional regulators, signaling proteins, cell cycle regulatory proteins, growth factors, and cytokines. Among them, thymus-expressed chemokine (TECK) and IP-30 were dramatically induced by Egr-1, but TNFalpha-related apoptosis inducing ligand (TRAIL) was significantly repressed by Egr-1, suggesting that Egr-1 is a key mediator of inflammation and apoptosis in vascular cells. These data provide novel Egr-1 target genes and contribute to the understanding of the role of Egr-1 in vasculature.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenoviridae / genetics
  • Apoptosis Regulatory Proteins
  • Blotting, Northern
  • Blotting, Western
  • Cell Line
  • Cell Line, Tumor
  • Chemokines, CC / genetics
  • Chemokines, CC / metabolism
  • DNA-Binding Proteins / genetics*
  • DNA-Binding Proteins / physiology
  • Down-Regulation
  • Early Growth Response Protein 1
  • Endothelium, Vascular / cytology
  • Endothelium, Vascular / metabolism*
  • Gene Expression Profiling
  • Gene Expression Regulation
  • Genetic Vectors / genetics
  • Humans
  • Immediate-Early Proteins*
  • Membrane Glycoproteins / genetics
  • Membrane Glycoproteins / metabolism
  • Oligonucleotide Array Sequence Analysis / methods
  • Oxidoreductases Acting on Sulfur Group Donors
  • Oxidoreductases*
  • Proteins / genetics
  • Proteins / metabolism
  • TNF-Related Apoptosis-Inducing Ligand
  • Transcription Factors / genetics*
  • Transcription Factors / physiology
  • Transfection
  • Tumor Necrosis Factor-alpha / genetics
  • Tumor Necrosis Factor-alpha / metabolism


  • Apoptosis Regulatory Proteins
  • CCL25 protein, human
  • Chemokines, CC
  • DNA-Binding Proteins
  • EGR1 protein, human
  • Early Growth Response Protein 1
  • Immediate-Early Proteins
  • Membrane Glycoproteins
  • Proteins
  • TNF-Related Apoptosis-Inducing Ligand
  • TNFSF10 protein, human
  • Transcription Factors
  • Tumor Necrosis Factor-alpha
  • Oxidoreductases
  • IFI30 protein, human
  • Oxidoreductases Acting on Sulfur Group Donors