Role of the RNA-binding protein HuR in colon carcinogenesis

Oncogene. 2003 Oct 16;22(46):7146-54. doi: 10.1038/sj.onc.1206862.


Immunohistochemical analysis of paired tumor and normal tissue specimens revealed that the expression and cytoplasmic abundance of the RNA-binding protein HuR increased with malignancy, particularly in colon carcinomas. Interventions to modulate HuR expression in human RKO colon cancer cells altered gene expression profiles and identified beta-catenin mRNA as a novel HuR target. Subcutaneous injection of HuR-overexpressing RKO cells into nude mice produced significantly larger tumors than those arising from control populations; conversely, RKO cells expressing reduced HuR through small interference RNA- or antisense HuR-based approaches developed significantly more slowly. We propose that HuR-regulated target mRNA expression contributes to colon cancer growth. Our results suggest a pivotal function for HuR in colon carcinogenesis.

MeSH terms

  • Animals
  • Antigens, Surface*
  • Base Sequence
  • Cell Nucleus / pathology
  • Cell Nucleus / physiology
  • Cell Transformation, Neoplastic / genetics
  • Colonic Neoplasms / genetics*
  • Colonic Neoplasms / pathology
  • Cytoplasm / pathology
  • Cytoplasm / physiology
  • ELAV Proteins
  • ELAV-Like Protein 1
  • Gene Expression Regulation, Neoplastic*
  • Humans
  • Mice
  • Mice, Nude
  • Oligonucleotide Array Sequence Analysis
  • RNA-Binding Proteins / genetics*
  • Templates, Genetic
  • Transcription, Genetic
  • Transfection
  • Transplantation, Heterologous
  • Tumor Cells, Cultured


  • Antigens, Surface
  • ELAV Proteins
  • ELAV-Like Protein 1
  • ELAVL1 protein, human
  • RNA-Binding Proteins