15d-PGJ2 inhibits cell growth and induces apoptosis of MCG-803 human gastric cancer cell line

World J Gastroenterol. 2003 Oct;9(10):2149-53. doi: 10.3748/wjg.v9.i10.2149.


Aim: To investigate the influence of peroxisome proliferator-activated receptor gamma (PPARgamma) ligand, 15-deoxy-12, 14-prostaglandin J2 (15dPGJ2) on the proliferation and apoptosis of MCG-803 human gastric cancer cell lines.

Methods: Cell proliferation was measured by 3H-TdR assay. Apoptosis was determined by ELISA and TUNEL staining. Protein and mRNA level of bcl-2 family and COXs were measured by Western blotting and Northern blotting respectively. PGE2 production was examined by RIA.

Results: 15dPGJ2 inhibited cell growth and induced apoptosis of MCG-803 cells. The COX-2 and bcl-2/bax ratios were decreased following 15dPGJ2 treatment. The PGE2 production in supernatants was also decreased. These changes were in a dose-dependent manner.

Conclusion: 15dPGJ2 may be a useful therapeutic agent for the treatment of gastric cancer.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Apoptosis / drug effects*
  • Cell Division / drug effects
  • Cell Line, Tumor
  • Cyclooxygenase 1
  • Cyclooxygenase 2
  • Dinoprostone / metabolism
  • Gene Expression Regulation, Neoplastic / drug effects
  • Humans
  • Isoenzymes / genetics
  • Membrane Proteins
  • Prostaglandin D2 / analogs & derivatives*
  • Prostaglandin D2 / pharmacology*
  • Prostaglandin-Endoperoxide Synthases / genetics
  • Proto-Oncogene Proteins / genetics
  • Proto-Oncogene Proteins c-bcl-2 / genetics
  • RNA, Messenger / analysis
  • Receptors, Cytoplasmic and Nuclear / genetics
  • Stomach Neoplasms*
  • Transcription Factors / genetics
  • bcl-2-Associated X Protein
  • bcl-X Protein


  • 15-deoxyprostaglandin J2
  • BAX protein, human
  • BCL2L1 protein, human
  • Isoenzymes
  • Membrane Proteins
  • Proto-Oncogene Proteins
  • Proto-Oncogene Proteins c-bcl-2
  • RNA, Messenger
  • Receptors, Cytoplasmic and Nuclear
  • Transcription Factors
  • bcl-2-Associated X Protein
  • bcl-X Protein
  • Cyclooxygenase 1
  • Cyclooxygenase 2
  • PTGS1 protein, human
  • PTGS2 protein, human
  • Prostaglandin-Endoperoxide Synthases
  • Dinoprostone
  • Prostaglandin D2