Blimp-1 is required for the formation of immunoglobulin secreting plasma cells and pre-plasma memory B cells

Immunity. 2003 Oct;19(4):607-20. doi: 10.1016/s1074-7613(03)00267-x.


Blimp-1 is a transcriptional repressor able to drive the terminal differentiation of B cells into Ig-secreting plasma cells. We have created mice with a B cell-specific deletion of prdm1, the gene encoding Blimp-1. B cell development and the number of B cells responding to antigen appear to be normal in these mice. However, in response to either TD or TI antigen, serum Ig, short-lived plasma cells, post-GC plasma cells, and plasma cells in a memory response are virtually absent, demonstrating that Blimp-1 is required for plasmacytic differentiation and Ig secretion. In the absence of Blimp-1, CD79b(+)B220(-) pre-plasma memory B cell development is also defective, providing evidence that this subset is an intermediate in plasma cell development. B cells lacking Blimp-1 cannot secrete Ig or induce muS mRNA when stimulated ex vivo. Furthermore, although prdm1-/- B cells fail to induce XBP-1, XBP-1 cannot rescue plasmacytic differentiation without Blimp-1.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cell Differentiation / physiology*
  • DNA-Binding Proteins / metabolism
  • Immunologic Memory / physiology*
  • Mice
  • Plasma Cells / physiology*
  • Positive Regulatory Domain I-Binding Factor 1
  • Regulatory Factor X Transcription Factors
  • Repressor Proteins / physiology*
  • Sequence Analysis, DNA
  • Time Factors
  • Transcription Factors / metabolism
  • Transcription Factors / physiology*
  • X-Box Binding Protein 1


  • DNA-Binding Proteins
  • Prdm1 protein, mouse
  • Regulatory Factor X Transcription Factors
  • Repressor Proteins
  • Transcription Factors
  • X-Box Binding Protein 1
  • Xbp1 protein, mouse
  • Positive Regulatory Domain I-Binding Factor 1