A transcriptional pause synchronizes translation with transcription in the tryptophanase operon leader region

J Bacteriol. 2003 Nov;185(21):6472-6. doi: 10.1128/JB.185.21.6472-6476.2003.

Abstract

Regulation of transcription of the tryptophanase operon requires that translation of its leader peptide coding region, tnaC, be coupled with its transcription. We show in vitro that a transcription pause site exists at the end of the tnaC coding region and that translation of tnaC releases the paused transcription complex, coupling transcription with translation.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Bacterial Proteins / genetics
  • Base Sequence
  • Escherichia coli Proteins / genetics
  • Molecular Sequence Data
  • Nucleic Acid Conformation
  • Operon*
  • Protein Biosynthesis*
  • Transcription, Genetic*
  • Tryptophanase / genetics*

Substances

  • Bacterial Proteins
  • Escherichia coli Proteins
  • tnaC protein, E coli
  • Tryptophanase