Evaluation of capillary HPLC/mass spectrometry as an alternative analysis method for gel electrophoresis of oligonucleotides

Nucleosides Nucleotides Nucleic Acids. 2003 May-Aug;22(5-8):1513-6. doi: 10.1081/NCN-120023023.

Abstract

A method has been developed to monitor the enzymatic incorporation of nucleotides in DNA by electrospray HPLC mass spectrometry. The main advantages of mass spectrometry over electrophoresis are the ability to directly characterize the reaction products and the shorter analysis time.

MeSH terms

  • Base Sequence
  • Capillary Action
  • Chromatography, High Pressure Liquid / methods
  • DNA / chemistry
  • DNA / isolation & purification
  • DNA Primers
  • Oligodeoxyribonucleotides / chemistry*
  • Oligodeoxyribonucleotides / isolation & purification
  • Spectrometry, Mass, Electrospray Ionization / methods
  • Templates, Genetic

Substances

  • DNA Primers
  • Oligodeoxyribonucleotides
  • DNA