Inhibition of gene expression by peptide nucleic acids in cultured cells

Nucleosides Nucleotides Nucleic Acids. 2003 May-Aug;22(5-8):1615-8. doi: 10.1081/NCN-120023081.

Abstract

We have tested in cultured cells the capacity of antisense and antigene PNAs to inhibit, in a sequence specific manner, the expression of oncogenes in leukaemia and pancreatic carcinoma cells. The results observed appeared promising and suggest that PNA may play in the future an important role in targeting disease-related genes.

MeSH terms

  • Animals
  • Base Sequence
  • Biological Transport
  • Cell Line
  • Codon / genetics
  • Gene Expression Regulation / drug effects*
  • Genes, ras / drug effects
  • Humans
  • Oligodeoxyribonucleotides, Antisense / pharmacokinetics
  • Oligodeoxyribonucleotides, Antisense / pharmacology
  • Peptide Nucleic Acids / pharmacokinetics
  • Peptide Nucleic Acids / pharmacology*
  • RNA, Messenger / genetics
  • Reverse Transcriptase Polymerase Chain Reaction
  • Tumor Cells, Cultured

Substances

  • Codon
  • Oligodeoxyribonucleotides, Antisense
  • Peptide Nucleic Acids
  • RNA, Messenger