Fluorescent complex of pyoverdin with aluminum

J Inorg Biochem. 2003 Dec 1;97(4):384-7. doi: 10.1016/s0162-0134(03)00316-7.

Abstract

When a pyoverdin (PV), (a siderophore) from Pseudomonas fluorescens, binds aluminum 1:1, its natural fluorescence almost doubles, whereas PV-Fe is non-fluorescent. Complex formation allows [Al] determination down to 1 mug/l. Fe(III) in the sample interferes with [Al] determination, but added after PV, improves the assay's performance. Ascorbic acid does not eliminate Fe(III) interference. PV-Al fluorescence could have analytical and toxicological applications.

MeSH terms

  • Aluminum / chemistry*
  • Ferric Compounds / chemistry
  • Fluorescence
  • Oligopeptides*
  • Pigments, Biological / chemistry*
  • Pseudomonas fluorescens / chemistry
  • Siderophores / chemistry

Substances

  • Ferric Compounds
  • Oligopeptides
  • Pigments, Biological
  • Siderophores
  • pyoverdin
  • Aluminum